Marmaduke (v. Owen Wilson) is a Great Dane living in rural Kansas with a cat named Carlos (v. George Lopez) His owner Phil (Lee Pace) works for Bark Organic dog food. Phil is very strict, from Marmaduke's perspective. One day, Carlos tells Marmaduke that he overheard Phil saying that they were being transferred to Orange County. They move from Kansas into their new house in California. Phil's boss, Don Twombly (William H Macy) has the goal of getting Bark Organic into every Petco store in the country. Phil and Don meet at the dog park to discuss Phil's assignment - an ad campaign to win over Petco - where Marmaduke meets a beautiful dog named Jezebel (v. Fergie), whose boyfriend is Bosco (v. Kiefer Sutherland), a controlling and violent dog with two minions named Thunder and Lighting. Bosco intimidates Marmaduke, who does not want to fight.